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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in many tumors, which ends up in chromosome instability, and thus might add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important purpose in advertising and marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data is not still accessible.
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Blocking peptides are peptides that bind particularly to your concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized via the antibody.
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This guideline outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This tutorial outlines further achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies sure to the blocking peptide no more bind on the epitope about the goal protein. By comparing the staining from the blocked antibody vs . the antibody by itself, one can see which staining is certain.
Focus on Facts Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation styles which add for the temporal coordination of each and every mitotic function. Cyclin E forms a fancy with and features as a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary which is degraded as cells progress through S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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